The Drosophila -Tubulin Small Complex Subunit Dgrip84 Is Required for Structural and Functional Integrity of the Spindle Apparatus□D
نویسندگان
چکیده
*Centre de Recherche en Pharmacologie-Santé, Unité Mixte de Recherche 2587, Centre National de la Recherche Scientifique-Pierre Fabre, Institut de Sciences et Technologies du Médicament de Toulouse, 31400 Toulouse, France; Centre de Biologie du Développement, Unité Mixte de Recherche 5547, Centre National de la Recherche Scientifique-Université Paul Sabatier, Institut d’Exploration Fonctionnelle des Génomes, 31062 Toulouse, France; †Instituto de Biologia Molecular e Celular, Universitade do Porto, 4150-180 Porto, Portugal; §Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4099-003 Porto, Portugal; and ‡Institut de Pharmacologie et de Biologie Structurale, Unité Mixte de Recherche 5089, 31077 Toulouse, France
منابع مشابه
The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus.
Gamma-tubulin, a protein critical for microtubule assembly, functions within multiprotein complexes. However, little is known about the respective role of gamma-tubulin partners in metazoans. For the first time in a multicellular organism, we have investigated the function of Dgrip84, the Drosophila orthologue of the Saccharomyces cerevisiae gamma-tubulin-associated protein Spc97p. Mutant analy...
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gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1- 12). Here, we report the purification and characterization of both complexes that we name gamma-tubulin small complex (gammaTuSC; approximately 280,000 D) and Drosophila gammaTuRC ( approximately 2,200,000 D). In addition to gamma-tubulin, th...
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g -tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1– 12). Here, we report the purification and characterization of both complexes that we name g -tubulin small complex ( g TuSC; z 280,000 D) and Drosophila g TuRC ( z 2,200,000 D). In addition to g -tubulin, the g TuSC contains Dgrip84 and Dgrip91,...
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